Peripheral Blood Mononuclear Cells from ATCC

Product Specs
ItemPeripheral Blood Mononuclear Cells
CompanyATCC
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPCS-800-011
Quantity1
TypePrimary Cell
SpeciesHuman
TissuePeripheral blood
Cell Type (standardized)Mononuclear Cells
Tissue (standardized)Blood
Applications3D cell culture, Immunology, Toxicology, Immuno-oncology, Cell and gene therapy (CGT) development
Research Area3D cell culture, Immunology, Toxicology, Immuno-oncology, Cell and gene therapy (CGT) development
Cell Typemononuclear
GenderLot specific
AgeLot specific
Diseasenormal

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) An Engineered Probiotic Produces a Type III Interferon IFNL1 and Reduces Inflammations in in vitro Inflammatory Bowel Disease ModelsACS Biomaterials Science & EngineeringSeptember 11, 2023Koon Jiew Chua, Hua Ling, In Young Hwang, Hui Ling Lee, John C. March, Yung Seng Lee, Matthew Wook ChangCO2 atmosphere. CD4+ T cells were isolated from human peripheral blood mononuclear cells (PBMCs, ATCC PCS-800-011) using an EasySep Human CD4+ T Cell Isolation Kit according to the manufacturer’s protocol. Isolated

(2) TAGAP expression influences CD4+ T cell differentiation, immune infiltration, and cytotoxicity in LUAD through the STAT pathway: implications for immunotherapyFrontiers in ImmunologySeptember 6, 2023Zhanyu Xu, Tiaozhan Zheng, Zhiwen Zheng, Wei Jiang, Liuliu Huang, Kun Deng, Liqiang Yuan, Fanglu Qin, Yu Sun, Junqi Qin, et al.Cell culture Peripheral blood mononuclear cells (hPBMC) from healthy individuals were purchased from ATCC (PCS-800-011). The Dynabeads® CD4 Positive Isolation Kit (cat. 11331D, Invitrogen™) was used for cell sorting, and

(3) Epithelial-Immune Cell Crosstalk Determines the Activation of Immune Cells In Vitro by the Human Cathelicidin LL-37 at Low Physiological ConcentrationsBiomoleculesAugust 28, 2023Ivan V. Bogdanov, Maria A. Streltsova, Elena I. Kovalenko, Alexander M. Sapozhnikov, Pavel V. Panteleev, Tatiana V. Ovchinnikovamononuclear cells (PBMCs) from a healthy donor were purchased from the American Type Culture Collection (ATCC PCS-800-011). Blood monocytes were isolated from PBMCs by plastic adhesion [17]. Mature monocyte-derived dendritic cells

(4) Immunomodulatory Effects of the Pea Defensin Psd1 in the Caco-2/Immune Cells Co-Culture upon Candida albicans InfectionInternational Journal of Molecular SciencesApril 23, 2023Ivan V. Bogdanov, Serafima I. Fateeva, Alexander D. Voropaev, Tatiana V. Ovchinnikova, Ekaterina I. Finkinacells (PBMCs) collected from healthy donors were purchased from American Type Culture Collection (ATCC PCS-800-011). Primary CD14+ blood monocytes were isolated from PBMCs by adherence according to standard technique

(5) Comparative and Temporal Characterization of LPS and Blue-Light-Induced TLR4 Signal Transduction and Gene Expression in Optogenetically Manipulated Endothelial CellsCellsFebruary 22, 2023Stierschneider A, Neuditschko B, Colleselli K, Hundsberger H, Herzog F, Wiesner C.University of Graz), and primary peripheral blood mononuclear cells (PBMCs) (ATCC®, Manassas, VD, USA; PCS-800-011™) (RRID:CVCL_WR41) were cultivated in a huMEC medium (InSCREENex, Braunschweig, Germany; INS-ME-1012)

(6) MTSS1 curtails lung adenocarcinoma immune evasion by promoting AIP4-mediated PD-L1 monoubiquitination and lysosomal degradationCell DiscoveryFebruary 21, 2023Yuan Wang, Zhenchang Jia, Chenxi Liang, Yunfei He, Min Cong, Qiuyao Wu, Pu Tian, Dasa He, Xiang Miao, Beibei Sun, et al.To analyze T cell-mediated tumor cell killing, human T cells were activated by culturing human PBMC (PCS-800-011, ATCC) in ImmunoCult-XF T cell expansion medium (10981, Stemcell) with ImmunoCult human CD3/CD28 T cell activator

(7) RNA cytometry of single-cells using semi-permeable microcapsulesNucleic Acids ResearchJanuary 11, 2023Greta Leonaviciene, Linas Mazutiscentrifugation steps were performed at 300 × g for 5 min at 4°C. Preparation of PBMCs PBMCs (ATCC, PCS-800–011) were thawed from liquid nitrogen using RPMI 1640 Medium supplemented with 10% fetal bovine serum. Thawed

(8) Antimicrobial Activity and Immunomodulatory Properties of Acidocin A, the Pediocin-like Bacteriocin with the Non-Canonical StructureMembranes (Basel)December 11, 2022Daria V. Antoshina, Sergey V. Balandin, Ivan V. Bogdanov, Maria A. Vershinina, Elvira V. Sheremeteva, Ilia Yu. Toropygin, Ekaterina I. Finkina, Tatiana V. Ovchinnikovaacute monocytic leukemia cell line (THP-1) were purchased from American Type Culture Collection (ATCC PCS-800-011 and ATCC TIB-202, respectively). PBMCs and THP-1 cells were seeded into 96-well plates at 2 × 106 and

(9) Antifungal Activity, Structural Stability, and Immunomodulatory Effects on Human Immune Cells of Defensin from the Lentil Lens culinarisMembranes (Basel)August 31, 2022Ekaterina I. Finkina, Ivan V. Bogdanov, Anastasia A. Ignatova, Marina D. Kanushkina, Ekaterina A. Egorova, Alexander D. Voropaev, Elena A. Stukacheva, Tatiana V. Ovchinnikovacells (PBMCs) collected from a healthy donor were purchased from American Type Culture Collection (ATCC PCS-800-011). CD4+ T-helper and CD8+ T cytotoxic cells were isolated from PBMCs by magnetic separation with mouse

(10) A Novel Proline-Rich Cathelicidin from the Alpaca Vicugna pacos with Potency to Combat Antibiotic-Resistant Bacteria: Mechanism of Action and the Functional Role of the C-Terminal RegionMembranes (Basel)May 12, 2022Pavel V. Panteleev, Victoria N. Safronova, Roman N. Kruglikov, Ilia A. Bolosov, Ivan V. Bogdanov, Tatiana V. Ovchinnikovaperipheral blood mononuclear cells (PBMC) collected from a healthy donor were purchased from American Type Culture Collection (ATCC PCS-800-011), thawed, and seeded into a 96-well plate one day prior to the experiment at a density of 2 × 105 cells/well.

(11) Immunomodulatory functions of human mesenchymal stromal cells are enhanced when cultured on HEP/COL multilayers supplemented with interferon-gammaMaterials Today BioJanuary 1, 2022Mahsa Haseli, David A. Castilla-Casadiego, Luis Pinzon-Herrera, Alexander Hillsley, Katherine A. Miranda-Munoz, Srikanth Sivaraman, Adrianne M. Rosales, Raj R. Rao, Jorge Almodovarcapacity, human primary peripheral blood mononuclear cells (PBMC), were purchased from ATCC (ATCC® PCS¬800¬011™ Part number: 302213). Briefly, 62500 hMSCs cells/cm2 with and without IFN-γ treatment were seeded on

(12) Preclinical validation of a live attenuated dermotropic Leishmania vaccine against vector transmitted fatal visceral leishmaniasisCommunications BiologyDecember 1, 2021Subir Karmakar et al.infection BMCs of seven different healthy donors from non-endemic regions were collected from ATCC, USA (PCS-800-011™). The viability of the PBMC was >98%, as checked by the trypan blue dye exclusion method. PBMCs were

(13) Characterizing the NLRP3 Inflammasome in Mood Disorders: Overview, Technical Development, and Measures of Peripheral Activation in Adolescent PatientsInternational Journal of Molecular SciencesNovember 19, 2021Xinyang Zhou, Shehan M. Fernando, Alexander Y. Pan, Rebecca Laposa, Kathryn R. Cullen, Bonnie Klimes-Dougan, Ana C. Andreazzautilized PBMC from unaffected individuals. Frozen PBMC from healthy donors (ATCC, Manassas, VA, USA, #PCS-800-011) were thawed and plated onto glass coverslips treated with poly-D-lysine (Fisher Scientific, Waltham,

(14) Therapeutic Potential of Chemically Modified, Synthetic, Triplex Peptide Nucleic Acid–Based Oncomir Inhibitors for Cancer TherapyCancer ResearchNovember 15, 2021Karishma Dhuri, Ravinder Reddy Gaddam, Ajit Vikram, Frank J. Slack, Raman Bahaltreatment control. Safety assessment by trypan blue assay Primary blood mononuclear cells (PBMC) (ATCC® PCS-800–011™) were purchased from ATCC (Virginia, USA). The cells were regularly tested for mycoplasma contamination

(15) Functional characterization of the immunomodulatory properties of human urine-derived stem cellsTranslational Andrology and UrologySeptember 1, 2021Rongpei Wu, Melisa Soland, Guihua Liu, Yingai Shi, Chi Zhang, Yiming Tang, Graça Almeida-Porada, Yuanyuan Zhangfollowing studies. Cell culture Human peripheral blood mononuclear cells (PBMNCs, normal human, ATCC PCS-800-011) from two different donors were purchased from ATCC (American Type Culture Collection, MD, USA). PBMNCs

(16) Antiviral, Cytotoxic, and Antioxidant Activities of Three Edible Agaricomycetes Mushrooms: Pleurotus columbinus, Pleurotus sajor-caju, and Agaricus bisporusJournal of FungiAugust 8, 2021Shaza M. Elhusseiny, Taghrid S. El-Mahdy, Mohamed F. Awad, Nooran S. Elleboudy, Mohamed M. S. Farag, Khaled M. Aboshanab, Mahmoud A. YassienUK). 2.6. Cell Lines and Cultures Primary peripheral blood mononuclear cells (PBMCs), normal, human (ATCC® PCS-800-011™) were purchased from Vascera, Cairo, Egypt. The cancer cell lines human leukemia (CCRF-CEM); acute promyelocytic leukemia

(17) Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible MushroomsMoleculesJuly 30, 2021Shaza M. Elhusseiny, Taghrid S. El-Mahdy, Mohamed F. Awad, Nooran S. Elleboudy, Mohamed M. S. Farag, Mahmoud A. Yassein, Khaled M. Aboshanab(Mokatam, Cairo, Egypt), while primary peripheral blood mononuclear cells (PBMCs) and normal human (ATCC® PCS-800-011™) cells were purchased from Vascera, Cairo, Egypt. This work was approved by the Ethical Research Committee

(18) A GATA3 Targeting Nucleic Acid Nanocapsule for In Vivo Gene Regulation in AsthmaACS NanoJuly 27, 2021Tyler D. Gavitt, Alyssa K. Hartmann, Shraddha S. Sawant, Arlind B. Mara, Steven M. Szczepanek, Jessica L. Rougefor p < 0.05. Cell Distribution Flow Cytometry. Human PBMCs were purchased from ATCC (catalog no. PCS-800-011). One million cells were aliquoted into each sample group and resuspended in FACS buffer. Groups receiving

(19) Human macrophages utilize a wide range of pathogen recognition receptors to recognize Legionella pneumophila, including Toll-Like Receptor 4 engaging Legionella lipopolysaccharide and the Toll-like Receptor 3 nucleic-acid sensorPLoS pathogensJuly 1, 2021Grigoryeva LS, Cianciotto NP.CCL-240), human primary macrophages derived from peripheral blood mononuclear cells (PBMC) (ATCC, #PCS-800-011), and murine primary macrophages derived from either the PBMCs or bone marrow (BMDMs) obtained from

(20) Investigation of Sensitization Potential of the Soybean Allergen Gly m 4 by Using Caco-2/Immune Cells Co-Culture ModelNutrientsJune 16, 2021Ivan V. Bogdanov, Ekaterina I. Finkina, Daria N. Melnikova, Rustam H. Ziganshin, Tatiana V. Ovchinnikovaperipheral blood mononuclear cells (PBMC) collected from healthy donor were purchased from American Type Culture Collection (ATCC PCS-800-011), thawed, and seeded into wells of 24- and 96-well plates 2 days prior to the experiment. Two different

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Peripheral Blood Mononuclear Cells from ATCC

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